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KMID : 0861020140290050055
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Korea Journal of Herbology
2014 Volume.29 No. 5 p.55 ~ p.63
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The effects of Danggwisusan on restoration ability in wound induced animal models
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Bak Ji-Won
Sim Boo-Yong
Kim Dong-Hee
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Abstract
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Objectives : The purpose of this study was verification of the restoration ability effect of Danggwisusan extract(DG) in wound induced Rat.
Methods : It needed to make a scar(around 2 × 2㎠) on the top of the fascia in the back of the rats and then the rats were divided into 4groups(n=6). Control was not treated at all, where as DG was orally medicated DG, Terra was percutaneously applied Terramycin, and DG + Terra was both orally medicated DG and percutaneously applied Terramycin per day for three weeks.
Results : 93% or higher cell viability was observed in all tested groups from 1, 10, 100 ㎍/㎖ in RAW 264.7cells. The DG decreased NO and cytokine production activity dose dependently. The production of IL-1β, IL-6 and TNF-α were decreased by 46%, 46% and 40% in DG treated 100 ㎍/㎖. The size of wound was significantly decreasing in DG, Terra, DG + Terra. WBC was significantly reduced in DG and DG + Terra. Monocyte was significantly reduced in DG, Terra and DG + Terra. Neutrophil was also reduced in DG, DG + Terra but not meaningless. The mRNA expression of MMP-1 was significantly reduced in Terra, MMP-2 was significantly reduced in DG, Terra, DG + Terra, and MMP-9 was significantly reduced in DG + Terra.
Conclusions : According to the results, we thought that DG showed anti-inflammatory activities on the RAW 264.7cells in mouse macrophage and in adult rat wound. Moreover, the progress of recovery was found visually, heamatologically, genetically and histopathologically. In conclusion, it could be thought that DG has effect on the treating of wound.
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KEYWORD
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Anti-inflammatory, Cyotokine, Danggwisusan, Matrix metalloprotease, MMP, Wound
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